In vitro cultivation of Ascaris lumbricoides var. suum larvae.

Ball (1953), Pitts and Ball (1955); Pitts (1960). The first two deal with the survival and growth in vitro of artificially hatched larvae while the last is concerned primarily with the growth of larvae axenized from mice. For the present investigation it was necessary to obtain large numbers of viable undamaged larvae. The methods previously reported for hatching larvae in vitro (Pitts, 1948; Haskins and Weinstein, 1957; Rogers, 1958) were examined and rejected because either the