HPLC fingerprint of Liuwei Dihuang condensed pills
2012
Objective: To establish HPLC fingerprints of Liuwei Dihuang condensed pills.Method: Dikma Diamonsil C18 column(4.6 mm×250 mm,5 μm) was adopted,with acetonitrile(containing 0.05% phosphoric)-water(containing 0.05% phosphoric) as the mobile phase.The column temperature was set at 40 ℃,and the flow rate was 1.0 mL·min-1.The detection wavelength was 276 nm(0-10 min),236 nm(10-40 min) and 276 nm(40-60 min).The sample size was 20 μL.Chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method.Result: Good precision,stability and repeatability were proved.Q-TOF-MS-IDA-MS/MS method was adopted for qualitative determination of eighteen chromatographic peaks.Ten batches of Liuwei Dihuang condensed pills were determined with the method,and their similarities were above 0.96.Conclusion: The study lays a foundation for the overall quality evaluation of Liuwei Dihuang condensed pills.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
3
Citations
NaN
KQI