Effects of Taxol pretreatment on oocytes vitrification of ovine.

Stabilizing the cytoskeleton system during vitrification is beneficial for improving the post-thaw survival and subsequent development of vitrified oocytes or embryos.As a cytoskeleton stabilizer,taxol increases the rate of polymerization by reducing the critical concentration of tubulin needed for polymerization,and it stabilizes microtubules by causing tighter links between alpha and beta tubulin dimers and by enhancing microtubular cross-linking.Therefore,the in vitro matured ovine oocytes was used as the material in this experiment,to determine the effect of vitrification on survival rate,normal morphylogy rate,embryo development rates after in vitro fertilization by pretreating in vitro mature ovine oocytes with taxol.After pretreating with the concentration of taxol at 0,0.5,1.0,1.5 μmol/L respectively,the oocytes were vitrified.Untreated oocytes were used as control.The results showed that the survival rate(82.38%)in 0.5 μmol/L taxol group was significantly higher(P 0.05) than those in other three groups;there was significant diferences between 0.5 μmol/L taxol group and control group in cleavage rate(51.37%vs.69.42%) and morula-blastocyst rate(20.30%vs.34.77%) of in vitro fertilization of frozen-thaw oocytes(P 0.05),but was significantly higher(P 0.05) than those in other three groups.In conclusion,0.5 μmol/L taxol pretreatment has positive effects on vitrified ovine oocytes matured in vitro.