Diketoreductase mutant and application thereof

The invention discloses a diketoreductase mutant and an application thereof. An amino acid sequence of the diketoreductase mutant is an amino acid sequence which is mutated from an amino acid sequence coded by a sequence shown in SEQ ID NO:9, the mutated amino acid sequence has at least two of the following mutation sites: a 94th bit, a 151st bit, a 231st bit, a 236th bit and a 251st bit, and I of the 94th bit is mutated into V, A or G; V of the 151st bit is mutated into Q, N or S; F of the 231st bit is mutated into W, Y or P; I of the 236th bit is mutated into L, V or A; and Q of the 251st bit is mutated into H, R or K; or the amino acid sequence of the diketoreductase mutant has mutation sites in the mutated amino acid sequence and more than 90% homogenous with the mutated amino acid sequence; and activity of the diketoreductase mutant with the mutation sites is greatly improved.