Çiftlik Hayvanlarında Brusellozisin Serolojik Tanısında Kullanılan Yöntemlerin Karşılaştırılması

In  this  study,  it  was  aimed  to  determine  the  sensitivity  and  comparative  analysis  of  serological  techniques  for  the  diagnosis  of  brucellosis  in  livestock.  One  hundred  cattle  and  100  sheep  with  a  history  of  abortion  both  of  that  have  not  been  vaccinated  against  brucellosis  along  with  100  horses  from  the  farms  of  traditional  management  in  Kars  region  of  Turkey  were  included  in  the  study,  with  a  cluster  sampling  method.  Brucella  antibodies  were  tested  through  Rose  Bengal  Plate  Test  (RBPT), Serum Agglutination Test (SAT) and Non-­‐Enzymatic Rapid Immunofiltration Assay (NERIFA). Of these, 1/40 and  above  for  the  cattle  and  horse  and  1/20  and  above  dilutions  for  sheep  were  considered  as  diagnostic  titre  in  the  SAT.  Out  of  100  cattle  samples,  26  (26%)  were  detected  positive  in  the  RBPT,  23  (23%)  in  the  SAT  and  25  (25%)  in  the  NERIFA  in  terms  of  Brucella  antibodies.  Out  of  100  sheep  sera  samples,  18  (18%)  with  the  RBPT,  14  (14%)  with  the  SAT  and  21  (21%)  with  the  NERIFA  were  found  positive  for  Brucella  antibodies.  For  horse  samples  13  (13%)  with  the  RBPT,  14  (14%)  with  the  SAT  and  12  (12%)  with  the  NERIFA  were  found  positive  for  Brucella  antibodies.  The  sensitivity  and  specifity  of  NERIFA  were  found  as  87%  and  93%  in  cattle,  85%  and  89%  in  sheep  and  78%  and  98%  in  horse.  The  agreement  between  the  NERIFA  and  the  RBPT  was found as almost perfectly as in cattle (kappa: 0.921) and horse (kappa: 0.862), while substantially in sheep (kappa:  0.713).  In  conclusion,  it  was  suggested  that  the  NERIFA  can  be  used  as  a  fast  screening  test  such  as  the  RBPT  for  diagnosis  of  Brucella  antibodies  in  farm  animals  both  in  the  field  and  in  limited  laboratory  conditions  and  the  present  results  are  needed  to  be  verified  with  more  advanced  techniques.