S_allele identification and genetic diversity analysis of apricot cultivars

ABSTRACTIn this study, the sexual incompatibility and S-allele diversity of 24 Turkish apricot cultivars, Paviot and Sakit-1 as parents and 127 F1 progenies were identified by polymerase chain reaction (PCR) and sequencing techniques. Additionally, genetic diversity and relatedness among the 24 cultivars were determined using 18 simple sequence repeat (SSR) markers from the genus Prunus. PCR for S_alleles identified nine different S-RNase alleles in the 24 apricot cultivars, namely Sc, S1, S2, S8, S9, S20, S24, S52, and S53. All primers amplified only one S_allele in the cultivars Adilcevaz-1, Adilcevaz-3, Ethembey, Pasamismisi, Canakkale, and Soganci. Most of the Turkish cultivars were self-incompatible. The Sc allele was present in only three cultivars (Canakkale, Ethembey, Imrahor) that are, therefore, self-compatible. The S_alleles of cultivars Paviot and Sakit-1 displayed homology with the Sc, S2 and S20, and S52 alleles. In the 127 F1 genotypes, the two S_alleles of Paviot were inherited by roughly ...