The potential fertility estimation capacity of the hypoosmotic swelling test, the thermal stress test and a modified cervical mucus penetration test in the bovine

Abstract In this study, hypoosmotic swelling (HOS), thermal stress (TS) and modified cervical mucus penetration (mCMP) tests have been used with routine tests for the assessment of semen quality. This is the first study in which the comparison of potential fertility estimation of fore-mention three tests was performed. Bull semen samples were divided into two fertility groups (high: n =3, low: n =3), according to their post-insemination NRR (non-return rate). Prior to the tests, post-thawed spermatological characteristics were assessed after which HOS, TS and mCMP tests were carried out. In the HOS test, the ratio of swollen cells, in the TS test the motility, and in the mCMP test the number of spermatozoa penetrating the cervical mucus, were examined. The relationship between the tests and fertility was also evaluated. HOS test was carried out according to different incubation times and temperatures (37°C 60min/41°C 15min/41°C 30min/46°C 15min/46°C 30min). For TS test, samples were subjected to various temperatures for different periods (no incubation (37°C)/41°C 15min/41°C 30min/46°C 15min/46°C 30min). The mCMP test were subjected to various temperatures for the same period (37°C 15min/41°C 15min). In this study, post-thawed motility was found to be similar in high and low fertility groups. However, it has been determined that acrosomal ( p p p p p