Preparation, isolation, and characterization of novel heterogeneous branched cyclomalto-oligosaccharides having β-d-galactosyl residue(s) on the side chain

Abstract Transgalactosylated products of branched cyclodextrins (glucosyl-αCD, -βCD, -γCD, and maltosyl-αCD, -βCD, -γCD) were synthesized by (β- d -galactosidases from Bacillus circulans and Penicillium multicolor using lactose as a donor substrate and branched CDs as acceptors. Eighteen (β- d -galactosylated branched CDs were isolated and purified by HPLC. Their structures were elucidated by FABMS and 13 C NMR spectroscopies, and methylation analysis. The chromatographic behavior of these novel heterogeneous branched CDs on three HPLC columns of different separation modes was compared.