Evaluación de cuatro métodos para la detección de Staphylococcus aureus meticilino-resistente de muestras clínicas en un hospital regional

Acosta-Perez G, Rodriguez-Abrego G, Longoria-Revilla E, Castro-Mussot ME. Evaluation of four methods for detecting methicillin-resistant Staphylococcus aureus isolates from clinical specimens at a regional hospital in Mexico. Salud Publica Mex 2012;54:1-6. Abstract Objetive.To estimate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in clinical isolates and to com- pare different methods for detection of MRSA in a lab with limited available personnel and resources. Material and Methods. 140 Staphylococcus aureus strains isolated from patients in several departments were assayed for β-lactamase production, MIC-Vitek 2 oxacillin, ChromID MRSA, disk dif- fusion in agar for cefoxitin 30 μg and PBP2a detection. The results of conventional tests were compared with the "gold standard" PCR test for mecA gene. Cohens kappa index was also calculated in order to evaluate the intra assay agreement between the used methods. Results. The found prevalence was 90.7%. Sensitivity and specificity were: disk diffusion for cefoxitin 97 and 92% respectively, MIC Vitek 2-XL 97 and 69%, ChromoID MRSA 97 and 85%, and PBP2a detection 98 and 100%. Conclusions. All methods are very good for detecting MRSA, choosing a method to use will depend on each laboratory infrastructure. Acosta-Perez G, Rodriguez-Abrego G, Longoria-Revilla E, Castro-Mussot ME. Evaluacion de cuatro metodos para la deteccion de Staphylococcus aureus meticilino-resistente de muestras clinicas en un hospital regional. Salud Publica Mex 2012;54:1-6 .