Towards a Multiplexed Barcode CRISPR/Cas12a-Assisted Platform for the Identification and Quantification of Single CpG Methylation Sites

Here we demonstrate a method to discriminate single CpG site methylation in DNA by the use of methylation-sensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing, which can be used as biomarker for several diseases. We show preliminary results of integrating CRISPR sensing in microfluidic channels for multiplexed “barcode” sensing of methylation markers.