Abstract 16123: Transcriptomic Profiling of Mitochondrial Dysfunction That Occurs in Arterial Calcification

Introduction: Arterial calcification(AC) is a significant contributor to cardiovascular mortality in patients with chronic kidney disease. We previously reported that heat shock protein 70 (HSP70) induction can inhibit vascular smooth muscle cell calcification. Mitochondrial complexes II, IV, NADH cytochrome c reductase, and cytochrome c oxidase activities were preserved by HSP70 induction leading to reduced apoptosis and RUNX2 expression. The molecular mechanisms of HSP70 vasculo-protective effects involving mitochondrial fusion regulation are currently unknown. Hypothesis: The goal of the present study was to investigate the transcriptomic profile of genes involved in mitochondrial function and vasculo-protective mechanisms of HSPs in AC. Methods: Human arteries were collected from healthy (n=15) and CKD (n=15) patients. AC was analyzed by Alizarin Red. Primary Human aortic endothelial cells (HAECs) was treated in calcification medium (CM: 5mM β-glycerolphosphate + 5mM CaCl2) in time-course experiments ...