ID: 16: Hydatid laminated layer from cestode helminth E. granulosus reduces severity in mice colitis through IFNγ, TNFα downregulation and IL10 upregulation

Inflammatory bowel disease is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effect of the laminated layer extracted from the cyst wall of a helminth parasite, Echinococcus granulosus , on dextran sulfate sodium (DSS)-induced colitis in mice. Crude extract of E. granulosus laminated layer was injected intra-peritoneally daily, starting 3 days before colitis induction. The Disease Activity Index was monitored daily and histological scores were evaluated. Nitric oxide (NO) and cytokine levels (interferon γ (IFN- γ ) , tumor necrosis factor α (TNF- α ) and interleukin 10 (IL-10)) were assessed. In addition, the colonic expression of inducible nitric oxide synthase (iNOS) and nuclear factor- κ B (NF- κ B) was examined. Importantly, our results indicate that hydatid laminated layer pretreatment significantly improved the clinical symptoms and histological scores observed during DSS-induced colitis. Furthermore, treatment with hydatid laminated layer caused a significant decrease in NO ( ∗∗ p 0.001 ), IFN - γ ( ∗ p α production ( ∗ p κ B expression. Our results demonstrate the potent protective effect of hydatid laminated layer in DSS induced colitis through downregulation of IFN- γ and TNF- α and up regulation IL-10 production. Furthermore, hydatid laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury suggesting the crucial role of helminth in immuno-protection against IBD.