THE ESR AND FLUOROMETRIC STUDIES ON INTERACTION OF NINE METAL IONS WITH HUMAN ERYTHROCYTE MEMBRANES

Objective:The present study deals with the interaction of metal ions with cell membrane and its relation to properties of metal ions. MethOds:By maleimide spin labeling ESR and N-[3-P]-M or fluorescamine labeled fluorometric method ,the interaction of metal ions Cd￣(2+),Ni￣(2+),Cu￣(2+),Pb￣(2+),Zn￣(2+),Co￣(2+),Mn￣(2+),Ca￣(2+),Ce￣(3+) with human erythrocyte membranes was studied.Results:The experiments in-dicated that the interaction between these metal ions and membranes was relative to hardness-softness of Lewis acid of the metal ions significantly.But Pb￣(2+) behaves like Ca￣(2+) on membranes as shown by spectroscopy,Ce￣(3+) behaves the same as Ca￣(2+); Mn￣(2+) induced considerable decrease of W/S value of la-beled membranes,somewhat like Cu￣(2+).Conclusion:The interaction of metal ions with human erythro-cyte membranes is ralative to the chemical properties of metal ions.