Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells

Wusheng Liu,Mary R. Rudis,Matthew H. Cheplick,Reginald J. Millwood,Jian-Ping Yang,Christine A. Ondzighi-Assoume,Garrett A. Montgomery,Kellie P. Burris,Mitra Mazarei,Jonathan D. Chesnut,Charles Neal Stewart

Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells

2019

Wusheng Liu
Mary R. Rudis
Matthew H. Cheplick
Reginald J. Millwood
Jian-Ping Yang
Christine A. Ondzighi-Assoume
Garrett A. Montgomery
Kellie P. Burris
Mitra Mazarei
Jonathan D. Chesnut
Charles Neal Stewart

Key message A novel and robust lipofection-mediated transfection approach for the use of DNA-free Cas9/gRNA RNP for gene editing has demonstrated efficacy in plant cells.

Keywords:

Plant cell
Cas9
Genetics
Biology
Lipofection
Genome editing
Ribonucleoprotein
DNA
Guide RNA
Cell biology
Fusion protein
Green fluorescent protein
CRISPR
Lipofectamine
Transfection

Correction
Source
Cite
Save
Machine Reading By IdeaReader

References

Citations