A novel class of sodium/calcium exchanger inhibitor: design, synthesis, and structure-activity relationships of 3,4-dihydro-2(1H)-quinazolinone derivatives.

Abstract Design, synthesis, and structure–activity relationships of 3,4-dihydro-2(1 H )-quinazolinone derivatives as inhibitors of the sodium/calcium (Na + /Ca 2+ ) exchanger are discussed. These studies, based on a lead compound 9a , which was identified in our library, involved systematic modification of three regions and revealed that (1) the 3,4-dihydro-2(1 H )-quinazolinone having a tertiary amino alkyl side chain at the 3-position is essential for activity, (2) a nonsubstituted phenyl ring is most suitable for high activity, and (3) introduction of a 4-substituted piperidine moiety enhanced the activity, in particular 4-benzylpiperidin-1-yl showed strong inhibitory activity. Based on these SAR studies, a structurally novel and highly potent inhibitor against the Na + /Ca 2+ exchanger, 12g ( SM-15811 ), was discovered. In particular, SM-15811 directly inhibited the Na + -dependent Ca 2+ influx via the Na + /Ca 2+ exchanger in cardiomyocytes with a high potency. The activity was almost two orders more potent than the lead compound 9a and SM-15811 exerted a protective effect against myocardial ischemic reperfusion injury. These Na + /Ca 2+ inhibitors could have a therapeutic potential for the treatment of ischemic reperfusion injury.