Spectroelectrochemical Characterization of Quinohemoprotein Alcohol Dehydrogenase from Gluconobacter suboxydans.

Direct electron transfer of alcohol dehydrogenase (ADH) from Gluconobacter suboxydans, which contains one pyrroloquinoline quinone (PQQ) and four hemes c, are realized on a carbon-wool column electrode. Potential-dependent spectral change monitored with a photodiode array detector is well explained by a five-step Nernstian electron transfer model. The evaluated redox potentials are reasonable to explain the chemical reactivity of ADH with various redox reagents.