Método de cromatografía líquida de alto rendimiento para la cuantifi cación de duloxetina en plasma de ratas High-performance liquid chromatography method for the quantifi cation of duloxetine in rat plasma

A sensitive and selective HPLC method was developed for quantifi cation of duloxetine, in rat plasma. Trifl uoperazine was used as an internal standard (IS). The present method used protein precipitation for extraction of the drug from rat plasma. Separation and quantifi cation was carried using in isocratic mode using 25 mM phosphate buffer (pH 3.0)/acetonitrile (60:40, % v/v) as mobile phase and on reverse-phase C 18 phenyl column (250 mm x 4.6 mm, 5µ) and the column effl uent was monitored by UV detector at 217 nm. This method was linear over the range of 44 - 2816.00 ng/ml with regression coeffi cient greater than 0.99. The mean recovery of duloxetine and IS were 82.33 ± 2.10 and 75.37 ± 1.07, respectively and the method was found to be precise, accurate and specifi c during the study. This validated method is sensitive and reproducible and it can be used for pharmacokinetic studies.