'Red pigment' from ADE-2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases.

Arturas Meškauskas,Vladimir N. Ksenzenko,Mikhail Shlyapnikov,Valentin Kryukov,Donaldas Čitavičius

'Red pigment' from ADE-2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases.

1985

Arturas Meškauskas
Vladimir N. Ksenzenko
Mikhail Shlyapnikov
Valentin Kryukov
Donaldas Čitavičius

Protection of DNA from cleavage by restriction endonucleases EcoRI, HindIII, BamHI, and Bg/II with red pigment, produced by ADE-2 mutants of Saccharomyces cerevisiae is demonstrated. Purification of yeast DNA from pigment can be achieved by chromatography on hydroxyapatite columns.

Keywords:

DNA
BamHI
HindIII
Yeast
Mutant
EcoRI
Saccharomyces cerevisiae
Biochemistry
Molecular biology
Restriction enzyme
Genetics
Biology
Cleavage (embryo)

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