Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system
2020
Pigeonpea [Cajanus cajan (L.) Millsp.] is a pulse crop cultivated in the semi-arid
regions of Asia and Africa. It is a rich source of protein and capable of alleviating
malnutrition, improving soil health and the livelihoods of small-holder farmers.
Hybrid breeding has provided remarkable improvements for pigeonpea productivity,
but owing to a tedious and costly seed production system, an alternative
two-line hybrid technology is being explored. In this regard, an environmentsensitive
male sterile line has been characterized as a thermosensitive male sterile
line in pigeonpea precisely responding to day temperature. The male sterile
and fertile anthers from five developmental stages were studied by integrating
transcriptomics, proteomics and metabolomics supported by precise phenotyping
and scanning electron microscopic study. Spatio-temporal analysis of anther
transcriptome and proteome revealed 17 repressed DEGs/DEPs in sterile anthers
that play a critical role in normal cell wall morphogenesis and tapetal cell development.
The male fertility to sterility transitionwasmainly due to a perturbation
in auxin homeostasis, leading to impaired cellwallmodification and sugar transport.
Limited nutrient utilization thus leads to microspore starvation in response
to moderately elevated day temperature which could be restored with auxin-treatment in the male sterile line. Our findings outline a molecular mechanism
that underpins fertility transition responses thereby providing a process-oriented
two-line hybrid breeding framework for pigeonpea.
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