Design of primers for pertussis diagnosis by Real Time PCR and determination of its sensitivity and specificity in comparison with commercial kits.

IJMM 1392; 7(3): P 1-7 Background and Aim: Pertussis vaccination in this country has been going on for many years and active infection or vaccination will provide immunity in 85% of cases. However, every 2-5 years outbreaks in unprotected adults creates an epidemic for children and infants. Based on conserved genomic sequences, Real time PCR could be an easy, costbenefit, fast and highly sensitive method for pertussis detection.