Rational Engineering of a Carboxylesterase for the Synthesis of Polyesters for Biomedical Applications

Reengineered variants of a hyperthermophilic carboxylesterase with improved product yield in the synthesis of poly(e-caprolactone) and triblock poly(e-caprolactone)-co-poly(ethylene glycol)Methods: Quantum Mechanics/Molecular Mechanics Molecular Dynamics simulations, enzyme expression, enzymatic assays and characterization of the products.