Abstract 39: Human Oral, Gut, and Blood Microbiota in Patients with Kawasaki Disease

Objective: We studied microbiome of throat swab, rectal swab, and venous blood obtained from patients with Kawasaki disease (KD) to determine whether bacterial nuclei are detectable in circulating blood of KD patients and whether the microbial composition resembles to that of oral cavity or gut. Methods: We initially studied 7 patients (4 males and 3 females, 20-59 months) and obtained swabs and blood samples before IVIG treatment (day 4-5) to prepare DNA and cDNA library. Next, we studied 14 patients (8 males and 6 females, 2-47 months) and prepared blood cDNA library before IVIG treatment (day 2-8) and before discharge (day 10-15). Samples were sequenced by Illumina HiSeq2000 and the results were analyzed using MePIC for human genome subtraction and megablast search, followed by taxonomic analysis with MEGAN viewer. Results: The number of sequences homologous to bacterial genome per swab sample obtained from DNA or cDNA libraries varied 20,000~4,700,000 or 500,000~2,400,000, respectively. In most of the...