Design and implementation of a microsensor platform for protein detection realized via 3-D printing

The possibility to implement engineered devices to obtain feedbacks from biological samples represent a powerful approach to bring significant improvement in diagnostics and biotechnological research. Among several 3D printing techniques, Aerosol Jet Printing (AJP) appears really promising for this kind of applications. Its main benefits are related to manufacturing of customized, complex geometries with a high resolution even on irregular surfaces. In this paper, we present the realization of an Aerosol Jet Printed microdevice addressed to protein detection and quantification in biological samples. The device was manufactured with particular attention to materials and geometry choices. A profilometer analysis was at first performed to determine the thickness of printed layers. Then electric tests were performed, to evaluate the electrical resistance offered by the printed elements. Finally, the possibility to immobilize antibodies on the sensor to perform protein quantification was assessed with fluorescence imaging. Results obtained confirmed the possibility to print with AJP high resolution lines, with proper values of resistivity. In conclusion, imaging findings suggested a good adhesion of antibodies on the electrodes, confirming the designed sensors as promising candidates for the electrochemical analysis of biological samples fluids.