Effects of T20N site-directed mutation on GTPase activities of OsRacD from Oryza sativa.

The experiment took chicken manure and pig manure to conduct composting experiment and researched on changes and interrelationships of enzyme activity and microbe quantity.The results showed that:the catalase activity and cellulose activity kept at a higher level in the early stage of composting,and then decreased quickly.Finally,the catalase activity between 9ml/g and 12ml/g,and the cellulose activity between 12.37mg/(kg·h)and 15.07mg/(kg·h).The trend of urease activity was "highed-lowed-highed".The trend of bacteria quantity was "low-high-low",actinomycetes quantity was "high-low" and fungi was "high-low-high".Through correlation analysis,the actinomycetes may was important factor that affected the change of catalase activity and cellulose activity.The actinomycetes had a significantly positive correlation with catalase in chicken manure,and had a positive correlation with catalase and cellulose in pig manure,and had a significantly positive correlation with catalase and cellulose in mixed chicken and pig manure.