Analysis of expression of the gene from the BRa puff of Chironomus thummi encoding the low molecular weight secretory protein

The plasmid containing F6.2 gene from the BRa of Chironomus thummi within the pUR 292 vector was constructed. Chimeric protein containing beta-galactosidase-F6.2 polypeptide was produced in Escherichia coli BMH71-18. The protein obtained has immunological similarity with secretion protein of 67,000 D from Ch. thummi salivary gland. The gene for sp67 is active in main and in special lobe of the gland. Based on the data obtained and on the previous results of the authors, conclusion is made that BRa is a complex locus containing several types of genes.