Biofabrication of Silver Oxide Nanoparticles (SO-NP) by autolysate of Pseudomonas mendocina PM1, and assessment of its antimicrobial/antibiofilm potential

Silver oxide Nanoparticles (SO-NP) exhibit excellent light absorbing, semi conducting properties and hence are employed in wide range of applications such as catalyst, biosensors, and in fuel cells. Green synthesis of nanoparticles using different microorganisms is a widely accepted since this method is cheap and eco-friendly. Nanoparticles synthesized by this route are smaller in size, highly stable, show high reactivity and stability. In this context, biofabrication of Silver Oxide Nanoparticles (SO-NP) by autolysate of Pseudomonas mendocina PM1 has been evaluated. Synthesis of SO-NP was observed, when autolysate of P. mendocina PM1 was incubated with 0.5 mM AgNO3 in dark for 24 h. Synthesis of SO-NP was confirmed by UV-Vis analysis. SO-NP was further confirmed by Transmission Electron Microscopy (TEM) and X-ray Diffraction (XRD) which confirmed presence of SO-NP. XRD revealed that SO-NP where of the type Ag3O4. FTIR analysis indicated that peptides were involved in the reduction and stability of SO-NP. SO-NP’s showed potent anti-microbial/ anti-biofilm activity against common pathogenic/non-pathogenic bacteria. This is the first report of synthesis of SO-NP by P. mendocina PM1