Cloning and preliminary analysis of promoter of rice glycosyl hydrolase gene GB27.

A glycosyl hydrolase gene GH27 which was associated with rice senescence was identified by bioinformation technology.The expression level of GH27 increased in late senescent leaves through Northern blot analysis which indicated the promoter PGH27 may be senescence specific.PGH27 was cloned using PCR method,and the sequence was analyzed.An expression vector containing the PGH27:GUS fusion gene was constructed and transferred into rice variety"Zhonghua 11"by Agrobacterium-mediated method.A strong expression of GUS was observed in both transgenic calli and senescing leaves.