2P-157 : Fluorescence on-off system silver hybrid-polymer dot for bacteria detection and killing

We studied an effective nanomaterial for the detection of bacteria and the measurement of antimicrobial activity using fluorescent polymer dots and silver nanoparticles. We developed a PVP@Ag:PD system that takes highly fluorescent form using catechol chemistry to avoid fluorescent reduction of PD-Ag due to FRET quenching. Positively charged PVP@Ag:PD promotes and aggregates with the bacteria surface which mostly negative charge, influencing the fluorescence of the system due to intermolecular interaction. Even at low concentrations, PVP@Ag:PD showed prominent and sensitive bacterial killing efficiency. Using this feature, we developed a system for detecting and killing bacteria that combines biosensors with antimicrobial activity. Thus, this system provides a possibility for future bacteria sensing and extermination. This was supported by Korea National University of Transportation in 2020.