Microbial community structure analysis in a hybrid membrane bioreactor via high-throughput sequencing.

Abstract Compared to the suspended culture (sludge) of a conventional bioreactor, hybrid membrane bioreactors (HMBRs) are more effective at removing pollutants owing to the addition of fillers addition of fillers that facilitate biofilm formation. However, the microbial community structure and composition in an HMBR remain unclear. We ran three laboratory-scale HMBRs at room temperature under different sludge retention times (SRTs; 10 d, 20 d, and 30 d) to compare the microbial diversity and community structure among the membrane surface, suspended filler surface, and mixed liquor using high-throughput sequencing. The results showed that SRT can markedly affect microbial community structure in the HMBR, and different trends appeared in the three functional units. The largest number of mutual operational taxonomic units was found in the activated sludge mixture and suspended carrier with an SRT of 20 d and 30 d. Species belonging to Proteobacteria in the 10 d SRT group had the greatest contribution to between-group differences. A longer SRT could mitigate membrane fouling by decreasing the relative abundance of Thauera and Sphaerotilus attached on the membrane surface. Comamonadaceae, a family of denitrifying bacteria, offer high denitrification potential for bioreactors operating under a long SRT.