Study on fusion of protoplasts from Sinorhizobium meliloti and Mesorhizobium ciceri

Streptomycin and penicillin were regarded as the sign of resistance to antibiotics of Sinorhizobium meliloti 102F28 and Mesorhizobium ciceri USDA3383 respectively.Using the protoplast fusion technique,102F28 and USDA3383 were successfully fused.Fusion hybrid can inoculate in the leguminous of parental strains respectively.There were apparent differences between parents and fusion hybrid in cell morphology,and pattern of whole-cell protein.The values of DNA homology between fusion hybrid and 102F28 and USDA3383 were 90.8% and 15.2% respectively.