Effect of Cytokinins on In Vitro Growth of Hypocotyl andCotyledon of Tomato (Lycopersicon esculentum)
2018
Study of regeneration from different tissues or organs of plants is important as it gives
information on how a piece of a plant can transform into its whole form. This process is even
substantial when we talk about genetic engineering in plants, since no genetic engineering
is valuable without knowing first the standard protocol for regenerating the transformed
tissue or organs to become a whole plant. This experiment used hypocotyl and cotyledon
of tomato cv. Tymoti as the explants was used to study how different concentrations (1.5-3
ppm) of cytokinins (Kinetin (Kin), 6-benzylaminopurine (BAP), thidiazuron (TDZ) and
Zeatin (Zn)) affect its growth. As many as 16 explants were used for each treatment. The
growth of both explants in the Murashige and Skoog (MS) media + vitamins showed that Zn
and TDZ were superior among the other treatments in inducing calli and primordia organ.
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