Effects of albumin and hypoxia on apoptosis of NRK-52E cells

Aim:To explore the effects of albumin and hypoxia co-stimulus on the apoptosis of tubular epithelial cell NRK-52E.Methods:Rat NRK-52E cell apoptosis rate was measured by flow cytometry after incubation by different albumin concentration(0,10,20,30,40 g/L) in normoxia(5% CO2 /95% N2) for 24 h to obtain appropriate albumin concentration.NRK-52E cell apoptosis rate was measured by flow cytometry after incubation in hypoxia(1% O2 /5% CO2 /94% N2) and albumin(30 g/L) for 24 h.The expressions of bcl-2 and bax mRNA were detected by RT-PCR.Results:NRK-52E apoptosis rate increased with the increase of albumin concentration in normoxia(F = 55.748,P 0.001).There were significant differences in NRK-52E apoptosis rate(F = 112.174,P 0.001),bax mRNA(F = 114.522,P 0.001) and bcl-2 mRNA(F =63.097,P 0.001) expression among normoxia group,hypoxia group,normoxia + albumin group and hypoxia + albumin group;NRK-52E apoptosis rate and bax mRNA expression increased significantly,however,bcl-2 mRNA expression decreased significantly in hypoxia + albumin group compared with that in normoxia + albumin group.Conclusion:NRK-52E cells apoptosis induced by albumin is aggravated by hypoxia,and bax and bcl-2 mRNA expression imbalance might involve in the potential mechanism.