Main bacterial groups in banana soil under rotated and continuous cropping

Banana wilt is the main disease in banana production,while banana-leek rotation can effectively control the occurrence of the disease.In order to understand the variations of soil bacterial groups under banana-leek rotation and banana continuous cropping,soil samples under these two cropping systems were collected to extract crude DNA,and the bacterial 16S rDNA in V3 region was amplified by PCR.The PCR products were then separated by DGGE,and the main different bands were sequenced and compared with the records of NCBI to identify the germs.Under banana-leek rotation,soil bacterial diversity was richer,and the main bacterial groups were Bacteroidetes,Proteobacteria,Actinobacteria,Chloroflexi,and Acidobacteria;while under banana continuous cropping,the soil bacterial diversity was somewhat decreased,and the main bacterial groups were Firmicutes,Proteobacteria,Actinobacteria,and Chloroflexi.