Blue-laser scanned imaging system using positioning marks formed on an optical disk substrate

We improved the optical disk-based imaging system to make more precise images at low cost for safety enhancement applications to observe and detect micrometer-order hazardous substances collected on a large-area surface. An optical disk substrate with positioning marks is prepared, and a signal processor is developed to automatically construct an image using the marks. We successfully obtained an image of Escherichia coli dispersed on an optical disk sample surface with a laser-scanning interval of 0.40 µm in the optical disk's radial direction; this interval is reduced from the 0.74 and 1.36 µm intervals demonstrated in our previous papers.