Problems in the determination of pancreatic enzymes for the quantitative functional diagnosis of the pancreas in children

: Pancreozymin-secretin tests were carried out in children aged from 0,5 till 12 years by means of a two lumen tube of the Salem-Sump-type and a three lumen perfusion tube. For stimulation we used 2 U/kg of the hormones, each from BOOTs-Corp. Amylase was determined with dinitrosalicylic acid, lipase by titration of acidic equivalents after half hour incubation and trypsin and chymotrypsin with TAME and BTEE respectively. We used PEG 4000 as marker and quantified enzymes secretion as well as liquid secretion by it. We got up to 50% lower results by testing with the two lumen tube. If we are laying the perfusion tube we measured an elevation of the two lumen tube. If we are laying the perfusion tube we measured an elevation of the II-hydroxycorticoides. The concentration of the enzymes in the specimines before perfusion on 3 consecutive days fell by 90% on the average. We discussed some problems of the determination of enzymes and performing of the tests by means of the results.