Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome.

Tumbarello, Buss and colleagues report that the motor protein myosin VI has an important role in autophagosome maturation. They show that myosin VI binds to autophagy adaptors to mediate delivery of endocytic cargo and endosomal membrane to autophagosomes, and promote autophagosome–lysosome fusion.