A tagging ALOX5AP polymorphism and risk of ischemic stroke in a northeastern Chinese Han population

Objective: 5-lipoxygenase-activating protein gene (ALOX5AP) has been recognized as a susceptibility gene for stroke. In this work, we explored the association of 6 ALOX5AP SNPs with cerebral infarction (CI) in a northeastern Chinese Han population, using a case-control design. Methods: A group of patients with cerebral infarction were randomly chosen as case group in northeastern Chinese Han population. Another comparative group of individuals without stroke were chosen as the control group. By utilizing TaqMan probe based real-time fluorescent PCR and DNA sequencing method, this study focused on 6 SNPs of ALOX5AP gene and analyzed the association with the hereditary susceptibility of cerebral infarction. Results: We found that, the rs9579646 G allele frequency was significantly associated with higher ischemic cerebral infarction. There was no significant difference of rs9551963, rs9315050, rs4769874, rs10507391 and rs4147064 genotype frequencies between the case and control group. Haplotype-based association analysis of the block involving rs9579646 and rs10507391 revealed that the increased risk of stroke was significantly associated with haplotype GT and GA. Conclusion: These results suggested that the genetic variants in ALOX5AP might be related to the risk of stroke in northeastern Chinese Han population. The SNP rs9579646 may be a diagnostic index of cerebral infarction.