Two Techniques for Simultaneous Identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by Use of the Small-Subunit rRNA Gene

The primers traditionally used to detect Plasmodium ovale infections are known for not binding all P. ovale parasites within the small-subunit rRNA gene when used alone. We describe a simple, cost- and time-efficient multiplex nested PCR and a nested PCR using a novel set of primers for the simultaneous detection of P. ovale curtisi and P. ovale wallikeri.