Up - regulation of cyclooxygenase - 2 in pancreatic carcinoma PC - 3 cell lines by cobalt chloride - stimulated hypoxia

Objective To investigate the expression of cyclooxygenase - 2 (COX- 2) and the effects of CoCl2 on the expression of cy-clooxygenase (COX - 2) in pancreatic carcinoma PC - 3 cell lines by inducing Hypoxia. Methods The expression of COX - 2 in pancreatic carcinoma PC- 3 cell lines was studied by immunohistochemistry. The time-concentration relationship and time-effect relationship between CoCl2 and COX - 2 were explored by reverse transcription polymerase chain reaction (RT - PCR). Results COX - 2 was expressed in pancreatic carcinoma PC - 3 cell lines. CoCl2 can up-regulate the expression of COX - 2 in PC - 3 cell lines in a time-dependent and dose-dependent manner. Conclusion COX - 2 was expressed in PC - 3 cell lines, and hypoxia can up-regulate the expression of COX - 2.