Gyrasen — biologische Werkzeuge zur Isolation bakterieller Nukleinsäuren

Targeted enrichment of DNA is often necessary for its detection in complex samples. We describe the development of a novel molecular tool for the specific enrichment of prokaryotic DNA. A fused protein comprising the DNA-binding subunit of the bacterial topoisomerase II, gyrase, was expressed and immobilized on magnetic particles. The specific affinity of the protein towards bacterial DNA in samples with high background of eukaryotic DNA was investigated.