清金得生片对人肺腺癌细胞（LAC）抑制与凋亡的诱导作用

【Objective】 To observe the effect of Qingjin Desheng Tablets (QDT) on inhibiting proliferation and inducing apoptosis of human lung adenocarcinoma cells (LAC). 【Methods】 Serum containing QDT at different concentrations (10%, 20% and 30% respectively) was obtained from SD rats which received gastric gavage of 8 g/kg QDT. Serum containing Tegafur (FT-207) at the concentration of 20% was obtained from SD rats which received gastric gavage of 0.2 g/kg FT-207. Meanwhile, the blank control serum was also prepared. The inhibitory effect of QDT on LAC proliferation was observed with methylthiazolyltetrazolium (MTT) assay. Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) double-labeled method and flow cytometry (FCM) were used to detect the early apoptosis of LAC, and the changes of LAC ultrastructure was examined under transmission electron microscope. 【Results】 Serum containing QDT inhibited LAC proliferation in a dose-time dependent manner, and the tumor-inhibitory rate of serum containing QDT at the concentration of 30% arrived 32% after 72-hour culture. Serum containing QDT also had an effect on inducing LAC apoptosis. And apoptotic features were obvious under transmission electron microscope in high-and moderate-dose groups, while not obvious in low-dose group. 【Conclusion】 The anti-tumor mechanism of QDT may be related to the inhibition of LAC proliferation and to the induction of LAC apoptosis.