[Rapid recognition and identification of chemical constituents in Qinghuo Zhimai tablets by SPE-HPLC-ESI-MS(n)].

Objective: To establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry(SPE-HPLC-ESI-MSn),in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets.Method: The possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MSn negative ion mode and by comparing with literature data or control samples.Result: Through the comparative analysis on Qinghuo Zhimai Tablets and components of its formula,39 chemical constituents were identified,including 7 caffeoylquinic acids,7 iridoids,6 diterpenoid lactones,5 homoisoflavonoids,13 steroidal saponins and 1 flavone glycoside.Conclusion: This study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets.