High-Performance Liquid Chromatographic Analysis of Amino Acid- and Peptide-Derived Chloramines

An isocratic reversed-phase high-performance liquid chromatographic method is reported for the analysis of amino acidand peptide-derived chloramines (these are important intermediates in two very different processes: destruction of microbes by the neutrophil and disinfection of water with chlorine). Specifically, results are reported for the chloramines derived from the following amino acids and dipeptides: taurine, Ala, Gly, Ser, Thr, Phe, Val, AlaGly, GlyGly, PheGly, SerGly, and ValGly. Analyses are performed on a 250×4.6-mm C 18 column using a buffered water-acetonitrile mixture as the mobile phase. For samples containing hydrophilic chloramines, an ion-pairing agent is added to the mobile phase. Two detection methods are used: direct ultraviolet (UV) detection of the chloramine at 254 nm and indirect UV detection of I3– at 350 nm