Establishment of the optimal RAPD reaction system of Setosphaeria turcica

The optimal RAPD reaction system of Setosphaeria turcica was established through experimentaion. In the total volume of 25 μL, there were 2 U Taq DNA polymerase, 2 mmol/L Mg~(2+), 200 μmol/L dNTP, 30 ng arbitrary primer, 1 μL 10×PCR buffer and 30 ng template DNA. Amplification was performed by the following program: 3 min at 94℃,40 cycles of 1min at 94℃, (1 min) at 37℃, 2 min at 72℃ and a final extention of 6 min at 72℃.