[Construction and identification of a recombinant lentiviral expression plasmid pLentiTrident1-hBMP2-Neo-hNGF].

PURPOSE: To construct and confirm a recombinant lentiviral vector containing human bone morphogenetic protein 2(hBMP2) and human nerve growth factor(hNGF).METHODS: The Neomycin gene was digested from pLentiTrident1-EGFP-Neo and then was subcloned into lentiviral vector.The hBMP2 and hNGF genes were amplified by polymerase chain reaction(PCR),and then the PCR product was inserted to proper sites of the vector.Finally,the recombinant vector pLentiTrident1-hBMP2-Neo-hNGF was confirmed by agarose gel electrophoresis and DNA sequence analysis.RESULTS: The construction of recombinant lentiviral vector pLentiTrident-hBMP2-Neo-hNGF was confirmed through restriction enzyme maping analysis and DNA sequencing.CONCLUSIONS: The recombinant lentiviral vector which can coexpress hBMP2 and hNGF is successfully constructed,which lays a solid foundation of studying the effect of neuro factors on bone regeneration.