Rapid Detection of Chromosomal Aneuploidy in Chorionic Villus of Spontaneous Abortion by Fluorescence in Situ Hybridization

Objective:To evaluate the application of fluorescence in situ hybridization(FISH) for the detection of chromosomal aneuploidy in chorionic villus of spontaneous abortion.Methods:Thirty cases of chorionic villus were analyzed by FISH,and seven probes were used to detect chromosomes 13,16,18,21,22,X,Y respectively.Results:In the thirty cases,seventeen cases were shown abnormal signals by FISH with detection rate of 57%,including eight cases of 16-trisomy,two cases of 22-trisomy,two cases of 13-trisomy and five cases of triploidy.Conclusions:FISH analysis is a rapid and easy method in the detection of chromosomal aneuploidy in chorionic villus.The application of FISH can provide important information of genetic counseling for the patients with spontaneous abortion.