Post-prandial exhaled 8-isoprostane responses to meals of varying caloric and fat content in non-asthmatic, insufficiently active men

INTRODUCTIONElevated levels of oxidative stress are associated with chronic diseases, such as asthma and cardiovascular disease. Even a single high-fat meal (HFM) leads to an increase in triglycerides, inflammation, and oxidative stress. In particular, 8-isoprostane, the gold standard for assessing oxidative stress, increases systemically (~48%) after a HFM in healthy men. However, no previous research has investigated whether airway oxidative stress increases after a HFM in non-asthmatics. Therefore, our purpose was to compare 8-isoprostane following two meals with differing levels of caloric and fat content in insufficiently active, non-asthmatic men. We hypothesized that airway 8-isoprostane would be significantly higher following a HFM as compared to a moderate-fat meal (MFM). METHODSEight non-asthmatic men (age: 25.8 ± 6.9 years, ≤30 minutes of exercise per week) completed two trials in a randomized cross-over design. Following a 10-hour fast, subjects consumed either a HFM (17 kcal/kg body mass, 60%...