Optical Control of Microdroplets for Cell-Encapsulating Polymerase Chain Reaction

Eric W. Hall,Kanaka Hettiarachchi,Hanyoup Kim,Gregory W. Faris

Optical Control of Microdroplets for Cell-Encapsulating Polymerase Chain Reaction

2014

Eric W. Hall
Kanaka Hettiarachchi
Hanyoup Kim
Gregory W. Faris

Laser heating for polymerase chain reaction (PCR) offers fast amplification times—15 min or less from cell lysis to assay completion. PCR is conducted for breast cancer cell DNA and calibrated via fluorescent oligo melting.

Keywords:

Cell
Lysis
DNA
Fluorescence microscope
Polymerase chain reaction
Biology
Molecular biology
optical control
Fluorescence
laser heating
Computer science

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