Chip as well as using method and application thereof

The invention relates to the biological detection field, in particular relates to the micro-fluidic chip field and relates to a chip as well as a using method and application thereof. The chip is provided with a reaction hole 303, a flow path 302 communicated with the reaction hole 303 and a sample adding hole 301, wherein the reaction hole 303 is arranged at the same side or at the two sides of the flow path 302, the reaction hole 303 and the flow path 302 are distributed in parallel, and the connecting position 501 of the reaction hole 303 and the flow path 302 is not higher than the lower edge of the reaction hole 303. The chip can enable the reaction liquid to be distributed into diffident reaction holes automatically and enable the reaction holes to be isolated with one another, and the liquid in different reaction holes can be recollected and is taken out of the chip after reaction through centrifugation.