Isolation and Quantification of Kaempferol-7-O-Glucoside and their Antimicrobial Screening of Cassia nodosa Bunch

Cassia species have been of keen interest in phytochemical and pharmacological research due to their excellent medicinal values. Different classes of na tural products, possessing potent physiological and pharmacological activities have been isolated from Cassia nodosa . They include anthracene derivatives, flavonoids and polysaccharides. Some of these compounds have been shown to possess considerable antimicrobial activity. The present study deals wit h the isolation, purification of flavonoids in diff erent parts of C. nodosa and their antimicrobial activity. A new flavonoid k aempferol-7-O-glucoside was identified and characterized and it showed significant antimicrobi al activities. The higher levels of total flavonoid s was measured in flowers of C. nodosa (1.15 mg/gdw) and, Similarly higher levels of tot al qurecetin (F+B) was measured in flowers of C. nodosa (0.34 mg/gdw). Higher levels of total kaempferol(F +B) was measured in flowers of C. nodosa (0.60 mg/gdw). Similarly higher levels of kaempfer ol-7-O-glucoside were measured in flowers of C. nodosa (0.21 mg/gdw). The isolated flavonoids were effecti ve against all test bacteria and fungi but the quercetin was found more effective against E. coli, A. flavus, A. niger, F. moniliformae and R. bataticola, therefore the MIC value 2◊10 3 mg/disc was recorded and MIC value for other bacteria and fungi was recorded as 3◊10 3 mg/disc. Kaempferol was more effective against A. flavus, A. niger, F. moniliformae and R. bataticola, therefore the MIC value 2◊10 3 mg/disc was recorded. MIC value for kaempferol-7-O-glucoside (a new flavonoid) was 2◊10 3 mg/disc for E. coli, A. flavus and A. niger but MIC value for S. aureus, P. aeurinosa and S. typhi was recorded 3◊10 3 mg/disc.